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Comprehensive Determination of Protein Tyrosine pK(a) Values for Photoactive Yellow Protein Using Indirect (13)C NMR Spectroscopy

Upon blue-light irradiation, the bacterium Halorhodospira halophila is able to modulate the activity of its flagellar motor and thereby evade potentially harmful UV radiation. The 14 kDa soluble cytosolic photoactive yellow protein (PYP) is believed to be the primary mediator of this photophobic res...

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Bibliographic Details
Main Authors: Oktaviani, Nur Alia, Pool, Trijntje J., Kamikubo, Hironari, Slager, Jelle, Scheek, Ruud M., Kataoka, Mikio, Mulder, Frans A.A.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2012
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3274806/
https://ncbi.nlm.nih.gov/pubmed/22325281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.12.024
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