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A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells.

The uvrA protein (UVRA) of E. coli has been extensively purified from a strain in which UVRA is overproduced and specifically labeled with 35S-methionine. This approximately 100-fold overproduction relative to normal strains is a result of having the uvrA gene present on a multicopy plasmid in a spr...

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Detaylı Bibliyografya
Asıl Yazarlar: Kacinski, B M, Sancar, A, Rupp, W D
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1981
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC327453/
https://ncbi.nlm.nih.gov/pubmed/6272208
Etiketler: Etiketle
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