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A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells.
The uvrA protein (UVRA) of E. coli has been extensively purified from a strain in which UVRA is overproduced and specifically labeled with 35S-methionine. This approximately 100-fold overproduction relative to normal strains is a result of having the uvrA gene present on a multicopy plasmid in a spr...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1981
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327453/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6272208/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.18.4495 |
| Oznake: |
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