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A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells.

The uvrA protein (UVRA) of E. coli has been extensively purified from a strain in which UVRA is overproduced and specifically labeled with 35S-methionine. This approximately 100-fold overproduction relative to normal strains is a result of having the uvrA gene present on a multicopy plasmid in a spr...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Kacinski, B M, Sancar, A, Rupp, W D
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1981
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327453/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6272208/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.18.4495
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