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Sedimentation velocity of DNA in isokinetic sucrose gradients: calibration against molecular weight using fragments of defined length.

The relationship between sedimentation coefficient and molecular weight for DNA sedimenting in preformed alkaline and neutral sucrose gradients was determined using absolute molecular weight standards (restriction fragments of plasmid pBR322 and phage lambda DNA). The range of calibration for alkali...

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Autores principales: Korba, B E, Hays, J B, Boehmer, S
Formato: Artigo
Lenguaje:Inglês
Publicado: 1981
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC327443/
https://ncbi.nlm.nih.gov/pubmed/6272206
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