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Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD
The production of the PEL polysaccharide in Pseudomonas aeruginosa requires the binding of bis-(3′,5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) to the cytoplasmic GGDEF domain of the inner membrane protein PelD. Here, the overexpression, purification and crystallization of a soluble constru...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
International Union of Crystallography
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3274398/ https://ncbi.nlm.nih.gov/pubmed/22297994 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111052109 |
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