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Ethidium bromide-mediated renaturation of denatured closed circular DNAs. The nature of denaturation-resistant fractions of bacteriophage PM2 closed circular DNA

Addition of the intercalating dye ethidium bromide (EtdBr) to a solution of alkali-denatured double-stranded closed circular PM2, ΦX174, or λb(2)b(5)c phage DNAs, under conditions such that the solution remains strongly alkaline, can result in the renaturation of up to 100% of the DNA upon neutraliz...

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Detalles Bibliográficos
Main Authors: Lau, Paul P., Gray, Horace B.
Formato: Artigo
Idioma:Inglês
Publicado: 1980
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC327299/
https://ncbi.nlm.nih.gov/pubmed/7443539
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