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In vitro splicing of SV40 late mRNA in isolated nuclei from CV-1 cells.

An in vitro splicing system utilizing isolated nuclei of SV40 infected cells has been developed. Nuclei were isolated from CV-1 cells at a late stage of SV40 infection after a pulse-labeling with 3H-uridine. In nuclei prepared under mild isotonic conditions, 19S viral coded RNA synthesized in vivo w...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphov: Hamada, H, Igarashi, T, Muramatsu, M
Materialtyp: Artigo
Språk:Inglês
Utgiven: Oxford University Press 1980
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327292/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6255447/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.3.587
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