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Quantitation in two-dimensional fluorescence difference gel electrophoresis: Effect of protein fixation

Analyzing a large number of unfixed gels in a 2-D fluorescence difference gel electrophoresis (2-DIGE) experiment presents a challenge of avoiding variable protein diffusion within and across the comparison groups. The characteristics of protein detection and quantitation in a 2-D differential in ge...

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Autori principali: Tannu, Nilesh, Hemby, Scott E.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3272766/
https://ncbi.nlm.nih.gov/pubmed/16607608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200500710
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