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Quantitation in two-dimensional fluorescence difference gel electrophoresis: Effect of protein fixation

Analyzing a large number of unfixed gels in a 2-D fluorescence difference gel electrophoresis (2-DIGE) experiment presents a challenge of avoiding variable protein diffusion within and across the comparison groups. The characteristics of protein detection and quantitation in a 2-D differential in ge...

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Detalhes bibliográficos
Main Authors: Tannu, Nilesh, Hemby, Scott E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3272766/
https://ncbi.nlm.nih.gov/pubmed/16607608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200500710
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