Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification

CPA is a class of isothermal amplification reactions that is carried out by a strand displacement DNA polymerase and does not require an initial denaturation step or the addition of a nicking enzyme. At the assay temperature of 63°C, the formation of a primer-template hybrid at transient, spontaneou...

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Autori principali: Xu, Gaolian, Hu, Lin, Zhong, Huayan, Wang, Hongying, Yusa, Sei-ichi, Weiss, Tristen C., Romaniuk, Paul J., Pickerill, Sam, You, Qimin
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2012
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3271364/
https://ncbi.nlm.nih.gov/pubmed/22355758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00246
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