A photoinduced cleavage of DNA useful for determining T residues.
Irradiation of 5'-[32P]-phosphate labeled DNA fragments with ultraviolet light in the presence of primary amines followed by piperidine treatment resulted in base-specific cleavage of the DNA chain at T residues, accompanied by a less intensive G reaction. This simple, T greater than G cleavage offe...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1982
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327062/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7162986/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.24.7959 |
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