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Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification.

The sequence of the Escherichia coli proC gene which encodes for delta 1-pyrroline-5-carboxylate (PCA) reductase was determined. Overproduction of the proC gene product via an expression plasmid carrying the bacteriophage lambda PL promoter allowed the purification to homogeneity of PCA reductase by...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Deutch, A H, Smith, C J, Rushlow, K E, Kretschmer, P J
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327040/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6296787/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.23.7701
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