Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification.

The sequence of the Escherichia coli proC gene which encodes for delta 1-pyrroline-5-carboxylate (PCA) reductase was determined. Overproduction of the proC gene product via an expression plasmid carrying the bacteriophage lambda PL promoter allowed the purification to homogeneity of PCA reductase by...

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Main Authors: Deutch, A H, Smith, C J, Rushlow, K E, Kretschmer, P J
Format: Artigo
Sprog:Inglês
Udgivet: 1982
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC327040/
https://ncbi.nlm.nih.gov/pubmed/6296787
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