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A general strategy for cloning double-stranded RNA: nucleotide sequence of the Simian-11 rotavirus gene 8.

Using Simian-11 rotavirus RNA, a strategy has been developed for the production of full length cloned copies of the genes of double-stranded (dsRNA) viruses. Genomic RNA segments were polyadenylated and reverse transcribed to yield a mixture of full length cDNA copies of both possible polarities. Th...

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Detalhes bibliográficos
Principais autores: Both, G W, Bellamy, A R, Street, J E, Siegman, L J
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC326989/
https://ncbi.nlm.nih.gov/pubmed/6296764
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