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Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization.
We present an improved procedure for detecting poly A tracts in situ by hybridization of 3H poly U. Glutaraldehyde fixation achieves significantly higher retention of RNA and better morphologic preservation than does Carnoy's. A dramatic increase in signal to noise is obtained by prehybridizati...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1981
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326896/ https://ncbi.nlm.nih.gov/pubmed/6169002 |
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