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Restriction enzyme digestion of hemimethylated DNA.
Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1981
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326867/ https://ncbi.nlm.nih.gov/pubmed/6269052 |
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