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Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit.

In the quaternary initiation complex, eIF-2.GMPPCP.Met-tRNAf.40S ribosomal subunit, the Met-tRNAf can be cross-linked to the beta subunit of initiation factor eIF-2 as well as to ribosomal proteins S3a and S6 by treatment with the bifunctional reagent, diepoxybutane. Using 40S subunits, modified in...

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Detalhes bibliográficos
Main Authors: Westermann, P, Nygård, O, Bielka, H
Formato: Artigo
Idioma:Inglês
Publicado em: 1981
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC326852/
https://ncbi.nlm.nih.gov/pubmed/6910637
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