Generation of monospecific antibodies based on affinity capture of polyclonal antibodies

A method is described to generate and validate antibodies based on mapping the linear epitopes of a polyclonal antibody followed by sequential epitope-specific capture using synthetic peptides. Polyclonal antibodies directed towards four proteins RBM3, SATB2, ANLN, and CNDP1, potentially involved in...

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Detalhes bibliográficos
Main Authors: Hjelm, Barbara, Forsström, Björn, Igel, Ulrika, Johannesson, Henrik, Stadler, Charlotte, Lundberg, Emma, Ponten, Fredrik, Sjöberg, Anna, Rockberg, Johan, Schwenk, Jochen M, Nilsson, Peter, Johansson, Christine, Uhlén, Mathias
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3267947/
https://ncbi.nlm.nih.gov/pubmed/21898641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.716
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