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Use of a cloned double stranded cDNA coding for a major androgen dependent protein in rat seminal vesicle secretion: the effect of testosterone in gene expression.

The abundant class of poly(A+)RNA [poly(A+)RNA11S] from rat seminal vesicle was used to synthesize ds-cDNA11S. The ds-cDNA11S was inserted and cloned into the Pst I site of pBR-322 using E. coli RR1 as host. Colony filter hybridization and restriction mapping was used to demonstrate that a 620 NTP l...

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Bibliografiset tiedot
Julkaisussa:Nucleic Acids Res
Päätekijät: Mansson, P E, Sugino, A, Harris, S E
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 1981
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326723/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6164983/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.4.935
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