Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis.

The subtilisin gene from B. amyloliquefaciens has been cloned and expressed under its own promoter on a high copy plasmid, pBS42, in Bacillus subtilis I-168 (Marburg strain). Greater than 95 percent of the expressed protease activity is secreted, and the activity is sensitive to inhibition by phenyl...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Wells, J A, Ferrari, E, Henner, D J, Estell, D A, Chen, E Y
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1983
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326549/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6316278/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.22.7911
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