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Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions.

The rates of blunt-end and cohesive-end ligation of DNA by T4 DNA ligase are increased by orders of magnitude in the presence of high concentrations of a variety of nonspecific polymers such as polyethylene glycol, Ficoll, bovine plasma albumin, or glycogen. Blunt-end ligation of small self-compleme...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Pheiffer, B H, Zimmerman, S B
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1983
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326544/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6359064/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.22.7853
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