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Enhanced expression of membrane proteins in E. coli with a P(BAD )promoter mutant: synergies with chaperone pathway engineering strategies
BACKGROUND: Membrane proteins (MPs) populate 20-30% of genomes sequenced to date and hold potential as therapeutic targets as well as for practical applications in bionanotechnology. However, MP toxicity and low yields in normally robust expression hosts such as E. coli has curtailed progress in our...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2011
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3265434/ https://ncbi.nlm.nih.gov/pubmed/22151946 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-105 |
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