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On the different binding affinities of CRP at the lac, gal and malT promoter regions.

We have determined the stoichiometry of CRP binding to various DNA fragments carrying the lac, malT or gal promoters in the presence of cAMP, using a gel electrophoresis method. In each case, one dimer of CRP binds to the functional CRP site upstream of the transcription start. At the lac promoter,...

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Bibliographic Details
Main Authors: Kolb, A, Spassky, A, Chapon, C, Blazy, B, Buc, H
Format: Artigo
Language:Inglês
Published: 1983
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC326541/
https://ncbi.nlm.nih.gov/pubmed/6316274
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