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Construction and characterization of type II collagen complementary deoxyribonucleic acid clones.
The mRNA for type II collagen was purified from embryonic chick sternum or from purified sternal chondrocytes with guanidine thiocyanate as the extractant. Double-stranded cDNAs to procollagen mRNAs from sternum were synthesized and dC-tailed. After annealing with PstI-cleaved, dG-tailed pBR322, thi...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1983
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326333/ https://ncbi.nlm.nih.gov/pubmed/6310524 |
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