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Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5' terminal domain of eukaryotic 28S rRNA.

We have developed a combined approach for probing native structures in large RNAs. In the first method, after digestion with a structure specific nuclease, accessible sites are mapped at sequence resolution along the entire RNA molecule which is used as a template for the reverse transcriptase elong...

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Bibliografiske detaljer
Main Authors: Qu, H L, Michot, B, Bachellerie, J P
Format: Artigo
Sprog:Inglês
Udgivet: 1983
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC326326/
https://ncbi.nlm.nih.gov/pubmed/6193488
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