An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid.
A strategy for kilo-base sequencing of a target DNA cloned in plasmid pWR34 is described. A long target DNA is progressively shortened from one end, by digestion with BAL31 nuclease or exonuclease III and nuclease S1, followed by cleaving off the shortened vector DNA. The family of the shortened tar...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1983
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326295/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6310503/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.16.5521 |
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