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Inhibition of Akt Activity and Calcium Channel Function Coordinately Drive Cell-Cell Fusion in the BeWO Choriocarcinoma Placental Cell Line

To establish a simple and quantitative live cell fusion assay for placental syncytialization, we generated stable GFP and dsRed expressing fusogenic BeWo cell lines. Fluorescent Activated Cell Sorting was shown to provide a quantitative determination of forskolin (cAMP-mediated) fusion in a time and...

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Detalhes bibliográficos
Main Authors: Vatish, Manu, Tesfa, Lydia, Grammatopoulos, Dimitris, Yamada, Eijiro, Bastie, Claire C., Pessin, Jeffrey E.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3261872/
https://ncbi.nlm.nih.gov/pubmed/22276109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0029353
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