Determination of the number of superhelical turns by the hyperchromicity of partially denatured covalently-closed DNA molecules.
A novel method of determining the number of superhelical turns of a covalently-closed plasmid DNA is described. It relies on the determination of the hyperchromicity, and hence the proportion of unstacked basepairs, of a partially heat-denatured sample which co-migrates during electrophoresis with n...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1982
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326155/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6895940/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.2.525 |
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