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Determination of the number of superhelical turns by the hyperchromicity of partially denatured covalently-closed DNA molecules.

A novel method of determining the number of superhelical turns of a covalently-closed plasmid DNA is described. It relies on the determination of the hyperchromicity, and hence the proportion of unstacked basepairs, of a partially heat-denatured sample which co-migrates during electrophoresis with n...

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Hlavní autoři: Dougherty, G, Koller, T
Médium: Artigo
Jazyk:Inglês
Vydáno: 1982
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC326155/
https://ncbi.nlm.nih.gov/pubmed/6895940
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