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Solubilization and immune-detection of beta-galactosidase hybrid proteins carrying foreign antigenic determinants.
Three DNA fragments representing almost the entire E1 gene of Semliki Forest virus (SFV) were inserted into a cro-lacZ expression vector by oligo dC.oligo dG tailing. Fragments inserted close to the 5' end of the lacZ gene gave rise to hybrid proteins which were rapidly degraded. Insertion of t...
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| Publicado no: | Nucleic Acids Res |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1983
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326026/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6191278/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.12.4077 |
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