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Modification of the anticodon triplet of E.coli tRNAMetf by replacement with trimers complementary to non-sense codons UAG and UAA.

E. coli tRNAMetf was hydrolyzed with RNase A using a limited amount of the enzyme to give two half molecules lacking the anticodon trimer and 3'-terminal dimer. Chemically synthesized trimers CUAp and UUAp were joined to the 5'-half molecules by phosphorylation with polynucleotide kinase p...

詳細記述

保存先:
書誌詳細
主要な著者: Ohtsuka, E, Doi, T, Fukumoto, R, Matsugi, J, Ikehara, M
フォーマット: Artigo
言語:Inglês
出版事項: 1983
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC326012/
https://ncbi.nlm.nih.gov/pubmed/6346266
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