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Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification

For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Using Primer Explorer software, four sets of primers were designed and RT-LAMP assay reaction conditions were optimized. The RT-LAMP was performed at...

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Main Authors: Zhang, Zong-Ying, Liu, Xiao-Jun, Li, Da-Wei, Yu, Jia-Lin, Han, Cheng-Gui
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3260119/
https://ncbi.nlm.nih.gov/pubmed/22185375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-8-550
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