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Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo.
Cloned genes have been purified from recombinant DNA bacteriophage libraries by a method exploiting homologous reciprocal recombination in vivo. In this method 'probe' sequences are inserted in a very small plasmid vector and introduced into recombination-proficient bacterial cells. Genomi...
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| Main Author: | |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1983
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325894/ https://ncbi.nlm.nih.gov/pubmed/6856467 |
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