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Highly Parallel and Short-Acting Amplification with Locus-Specific Primers to Detect Single Nucleotide Polymorphisms by the DigiTag2 Assay

The DigiTag2 assay enables analysis of a set of 96 SNPs using Kapa 2GFast HotStart DNA polymerase with a new protocol that has a total running time of about 7 hours, which is 6 hours shorter than the previous protocol. Quality parameters (conversion rate, call rate, reproducibility and concordance)...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Nishida, Nao, Mawatari, Yoriko, Sageshima, Megumi, Tokunaga, Katsushi
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Public Library of Science 2012
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3258256/
https://ncbi.nlm.nih.gov/pubmed/22253840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0029967
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