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Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR

Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples, the possible presence of inhibitors forms a c...

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Autori principali: Lievens, Antoon, Van Aelst, S., Van den Bulcke, M., Goetghebeur, E.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3258155/
https://ncbi.nlm.nih.gov/pubmed/22102586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr775
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