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Characterization of a prokaryotic topoisomerase I activity in chloroplast extracts from spinach.

A topoisomerase I activity has been partially purified from crude extracts of spinach chloroplasts. This activity relaxes the supercoiled covalently closed circular DNA of pBR322. The enzyme requires Mg++, but not ATP, and has an apparent molecular weight of about 115,000. It catalyzes a unit change...

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Bibliografiske detaljer
Main Authors: Siedlecki, J, Zimmermann, W, Weissbach, A
Format: Artigo
Sprog:Inglês
Udgivet: 1983
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC325812/
https://ncbi.nlm.nih.gov/pubmed/6298746
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