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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. T...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1983
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325809/ https://ncbi.nlm.nih.gov/pubmed/6828386 |
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