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Identification of clones carrying an E. coli tRNAPhe gene by suppression of phenylalanyl-tRNA synthetase thermosensitive mutants.

Two libraries of cloned E. coli DNA were screened for plasmids which complemented thermosensitive phenylalanyl-tRNA synthetase mutants. Four plasmids were isolated which complemented pheS and pheT thermosensitive mutations but which do not carry pheS or pheT, the structural genes for phenylalanyl-tR...

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Publicat a:Nucleic Acids Res
Autors principals: Caillet, J, Plumbridge, J A, Springer, M, Vacher, J, Delamarche, C, Buckingham, R H, Grunberg-Manago, M
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1983
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC325749/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6300764/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.3.727
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