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Crosslinking of Escherichia coli 50S ribosomal subunits with chlorambucilyl oligoprolyl phenylalanyl-tRNA molecular rulers.

A series of P-site probes, chlorambucilyl-(Pro)n-Phe-tRNAPhe, were prepared and reacted with poly(U)-directed Escherichia coli MRE 600 ribosomes. Upon binding of the probes to ribosomes, 90% of the cpm bound were not released following subsequent interaction with puromycin. In the absence of poly(U)...

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Autors principals: Parker, K K, Wickstrom, E
Format: Artigo
Idioma:Inglês
Publicat: 1983
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC325730/
https://ncbi.nlm.nih.gov/pubmed/6338479
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