Importance of the Protein Framework for Catalytic Activity of [FeFe]-Hydrogenases

The active center (H-cluster) of [FeFe]-hydrogenases is embedded into a hydrophobic pocket within the protein. We analyzed several amino acids, located in the vicinity of this niche, by site-directed mutagenesis of the [FeFe]-hydrogenases from Clostridium pasteurianum (CpI) and Chlamydomonas reinhar...

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Autors principals: Knörzer, Philipp, Silakov, Alexey, Foster, Carina E., Armstrong, Fraser A., Lubitz, Wolfgang, Happe, Thomas
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2012
Matèries:
Molecular Biophysics
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3256906/
https://ncbi.nlm.nih.gov/pubmed/22110126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.305797
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