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Importance of the Protein Framework for Catalytic Activity of [FeFe]-Hydrogenases
The active center (H-cluster) of [FeFe]-hydrogenases is embedded into a hydrophobic pocket within the protein. We analyzed several amino acids, located in the vicinity of this niche, by site-directed mutagenesis of the [FeFe]-hydrogenases from Clostridium pasteurianum (CpI) and Chlamydomonas reinhar...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Biochemistry and Molecular Biology
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3256906/ https://ncbi.nlm.nih.gov/pubmed/22110126 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.305797 |
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