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Purification, Characterization, and Expression of Multiple Glutamine Synthetases from Prevotella ruminicola 23

The Prevotella ruminicola 23 genome encodes three different glutamine synthetase (GS) enzymes: glutamine synthetase I (GSI) (ORF02151), GSIII-1 (ORF01459), and GSIII-2 (ORF02034). GSI, GSIII-1, and GSIII-2 have each been heterologously expressed in and purified from Escherichia coli. The subunit mol...

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Detalhes bibliográficos
Main Authors: Kim, Jong Nam, Cann, Isaac K. O., Mackie, Roderick I.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3256599/
https://ncbi.nlm.nih.gov/pubmed/22020637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.05916-11
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