Getting the measure of syphilis: qPCR to better understand early infection

OBJECTIVES: Until recently, PCR had been used to detect but not quantify Treponema pallidum. To understand infection kinetics of this uncultivable organism, a real-time PCR assay was developed to quantify 47 kDa membrane lipoprotein gene DNA (tpp47). METHODS: Assay specificity was determined against...

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Detalhes bibliográficos
Main Authors: Tipple, Craig, Hanna, Mariam O F, Hill, Samantha, Daniel, Jessica, Goldmeier, David, McClure, Myra O, Taylor, Graham P
Formato: Artigo
Idioma:Inglês
Publicado em: BMJ Group 2011
Assuntos:
Basic Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3252622/
https://ncbi.nlm.nih.gov/pubmed/21752804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1136/sti.2011.049494
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