Towards a rapid molecular diagnostic for melioidosis: comparison of DNA extraction methods from clinical specimens

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was su...

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Main Authors: Richardson, Leisha J, Kaestli, Mirjam, Mayo, Mark, Bowers, Jolene R, Tuanyok, Apichai, Schupp, Jim, Engelthaler, David, Wagner, David M, Keim, Paul S, Currie, Bart J
Format: Artigo
Sprog:Inglês
Udgivet: 2011
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3249147/
https://ncbi.nlm.nih.gov/pubmed/22108495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2011.10.023
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