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A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces

We demonstrate a novel and robust microfluidic chip with combined functions of continuous culture and output of PC-3 prostate cancer cells. With digital controls, polydimethylsiloxane (PDMS) flexible diaphragms are able to apply hydrodynamic shear forces on cultures, detaching a fraction of attached...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Liu, Liyu, Loutherback, Kevin, Liao, David, Yeater, David, Lambert, Guillaume, Estévez-Torres, André, Sturm, James C., Getzenberg, Robert H., Austin, Robert H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3248645/
https://ncbi.nlm.nih.gov/pubmed/20424729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c003509b
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