The epitope for the polyol-responsive monoclonal antibody 8RB13 is in the flap-domain of the beta-subunit of bacterial RNA polymerase and can be used as an epitope tag for immunoaffinity chromatography

Polyol-responsive monoclonal antibodies (PR-mAbs) are useful for the purification of proteins in an easy, one step immunoaffinity step. These antibodies allow for gentle purification of proteins and protein complexes using a combination of a low molecular weight polyhydroxylated compound (polyol) an...

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Bibliografiset tiedot
Päätekijät: Stalder, Elizabeth S., Nagy, Lauren H., Batalla, Pilar, Arthur, Terrance M., Thompson, Nancy E., Burgess, Richard R.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2011
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Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3248238/
https://ncbi.nlm.nih.gov/pubmed/21215316
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2010.12.011
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