Preparation of high-quality next-generation sequencing libraries from picogram quantities of target DNA

New sequencing technologies can address diverse biomedical questions but are limited by a minimum required DNA input of typically 1 μg. We describe how sequencing libraries can be reproducibly created from 20 pg of input DNA using a modified transpososome-mediated fragmentation technique. Resulting...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Parkinson, Nicholas J., Maslau, Siarhei, Ferneyhough, Ben, Zhang, Gang, Gregory, Lorna, Buck, David, Ragoussis, Jiannis, Ponting, Chris P., Fischer, Michael D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2012
Soggetti:
Method
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3246199/
https://ncbi.nlm.nih.gov/pubmed/22090378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.124016.111
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi