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Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA.
We have developed a method to accurately determine (< 3% RSD) the complete major and modified base composition of a few micrograms of unlabeled DNA. The DNA samples were quantitatively hydrolyzed with DNase 1, Nuclease P1, and bacterial alkaline phosphatase. The resulting deoxyribonucleosides wer...
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| Auteurs principaux: | , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1980
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC324386/ https://ncbi.nlm.nih.gov/pubmed/7003544 |
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