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2'5' oligoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2'5' oligoadenylates and 2'5' co-oligonucleotides.

The interferon induced enzyme 2'5' oligoadenylate synthetase produces 2'5' pppA(pA)n the first discovered natural nucleotide with a 2'5' linkage. We describe a direct assay of this enzyme based on separation by thin layer chromatography (TLC) of the substrate ATP and the products 2'5' pppA(pA)n (n l...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Justesen, J, Ferbus, D, Thang, M N
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1980
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC324356/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6160465/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.14.3073
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