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A subcloning strategy for DNA sequence analysis.
We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid....
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| Publicado no: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1980
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC324324/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6258146/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.23.5541 |
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