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Selective 32P-labelling of individual species in a total tRNA population.
A simple procedure to label individual tRNA species in a total tRNA preparation has been developed. The principle of the method is as follows: total crude tRNA (from E. coli) is incubated in the presence of a crude aminoacyl-tRNA synthetase preparation, containing most aminoacyl-tRNA synthetases and...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1980
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC324297/ https://ncbi.nlm.nih.gov/pubmed/7008026 |
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