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Site-specific DNA-affinity chromatography of the lac repressor.
To test the feasibility of site-specific DNA-affinity chromatography, E. coli lac repressor was bound to an operator-containing DNA column, and in parallel to a non-operator DNA column. Salt gradient elution shows: 1) elution from non-operator DNA was near 250mM KCl or NaCl; interpretation of this r...
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| Glavni autor: | |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1980
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| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC324186/ https://ncbi.nlm.nih.gov/pubmed/7001362 |
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