Site-specific DNA-affinity chromatography of the lac repressor.
To test the feasibility of site-specific DNA-affinity chromatography, E. coli lac repressor was bound to an operator-containing DNA column, and in parallel to a non-operator DNA column. Salt gradient elution shows: 1) elution from non-operator DNA was near 250mM KCl or NaCl; interpretation of this r...
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| Udgivet i: | Nucleic Acids Res |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1980
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC324186/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7001362/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.16.3721 |
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