Viability assessment of fresh and frozen/thawed isolated human follicles: reliability of two methods (Trypan blue and Calcein AM/ethidium homodimer-1)

PURPOSE: To assess the reliability of trypan blue (TB) and calcein AM/ethidium homodimer-1 (CaAM/EthD-1) staining to evaluate the viability of fresh and thawed human ovarian follicles. METHODS: Isolated follicles from fresh and thawed cortex were stained using TB versus CaAM/EthD-1 methods (n = 10 p...

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Main Authors: Sanfilippo, Sandra, Canis, Michel, Ouchchane, Lemlih, Botchorishvili, Revaz, Artonne, Christine, Janny, Laurent, Brugnon, Florence
Formato: Artigo
Idioma:Inglês
Publicado: Springer US 2011
Assuntos:
Fertility Preservation
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3241833/
https://ncbi.nlm.nih.gov/pubmed/22033548
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-011-9649-y
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