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Cloning and sequence of the cDNA corresponding to the variable region of immunoglobulin heavy chain MPC11.

Poly(A)-containing mRNA from mouse myeloma MPC11 was transcribed into cDNA which was cloned in the PstI site of the plasmid pBR322. The transformants were screened by hybridization with a cDNA fragment, derived from plasmid p gamma(11)7, corresponding to the 5' portion of the constant region of...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Zakut, R, Cohen, J, Givol, D
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1980
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC324177/
https://ncbi.nlm.nih.gov/pubmed/6253904
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