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An improved direct RNA sequence method; its application to Vicia faba 5.8S ribosomal RNA.
We have developed a direct read-off sequencing procedure, based on the method of Stanley and Vassilenko using E. coli 5S ribosomal RNA as a model compound. Radioactive bands were transferred from an acrylamide gel fractionation in the first dimension onto a DEAE-cellulose thin layer plate. After in...
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| Main Authors: | , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1980
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC323990/ https://ncbi.nlm.nih.gov/pubmed/6159591 |
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